Tiger, Trajectory Inference from Gene Expression Reference, provides the inference of cell diferentiation pathways. Through Tiger module, mechanisms of regulation of cell differentiation, timelines and movement trajectories could be explored. we calculated the stemness of each cell and predicted genes positively or negatively correlated with stemness, therefore screening putative promoter or inhibitor of cell differentiation. For instance, in the dataset BS0000205 (human primary glioblastoma sample), the stemness of endothelial, astrocytes, neurons, OPC, Olg was predicted to be in descending order.