Integrating a priori knowledge and newly generated resources across 100 vertebrates, providing an unprecedented reference for investigating brain evolution.
Cross-species single-nucleus transcriptomic data from 100 vertebrates, including 50 newly sequenced species and 50 public datasets, covering major vertebrate lineages.
Gene-level, synteny-based (SBP) gene-age analysis across 11,578 cross-species-retained orthologs shows that the analysis layer is 86.7% euteleostome-stem-or-older, and that 40 of 45 aged core cell-type markers trace to the euteleostome stem or deeper, the remainder being young duplicates of ancient families.
A curated catalog of 43 atlas-level clusters, each spanning a median of 99 of the 100 species, with 98.3% of the 492,121 analysis-layer cells receiving unified cell-type annotations.
Access our cross-species reference freely and accelerate your research in brain evolution and precision medicine.